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Biomedical Engineering Consulting
Biomedical Engineering Consulting
Molecular Biology
Primer/Plasmid design, cloning, site-directed mutagenesis, plasmid purification, PCR, RT-PC
Protein-protein / Protein-ligand Characterization
Fluorescence anisotropy/polarization, ITC, DSC, SPR
BLI/Octet, immunoprecipitation/pulldown assays
Macromolecular Crystallography
High-throughput screening (TTP LabTech, Formulatrix), crystal optimization, cryopreservation
X-ray diffraction, data collection and processing
Molecular replacement, SAD, MAD
Computational Biology
Virtual screening, DOCK, AMBER, MD/Monte Carlo simulations, MOE Molecular replacement, SAD, MAD
Primer/Plasmid Design, Cloning, Site-directed Mutagenesis, Plasmid purification, PCR, RT-PC
Bacterial, insect, and mammalian cell lines
Isotopic labeling (13C, 15N) of proteins
HPLC, FPLC, and batch purification strategies
Thermal (thermofluor) and chemical denaturation of proteins, circular dichroism
Additional Expertise
Multi-color flow cytometry, mammalian cell culture, purification of mitochondria
Fluorescence microscopy, MitoTracker/Calcein release assays
Partner with Us
Electrical Engineering Consultant
(619) 877 4232
rich@heinzeconsultants.com
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